cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.
Identifieur interne : 000559 ( Main/Exploration ); précédent : 000558; suivant : 000560cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.
Auteurs : R A Hooft Van Huijsduijnen [Pays-Bas] ; L C Van Loon ; J F BolSource :
- The EMBO journal [ 0261-4189 ] ; 1986.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ARN des plantes (génétique), ARN messager (génétique), Biosynthèse des protéines (MeSH), Clonage moléculaire (MeSH), Hybridation d'acides nucléiques (MeSH), Protéines végétales (génétique), Tabac (virologie), Technique de Northern (MeSH), Virus de la mosaïque du tabac (génétique).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Complementary, Plant Proteins, RNA, Messenger, RNA, Plant.
- genetics : Tobacco Mosaic Virus.
- virology : Tobacco.
- Blotting, Northern, Cloning, Molecular, Nucleic Acid Hybridization, Protein Biosynthesis.
Abstract
A cDNA library was constructed to 10-15 S poly(A) RNA from tobacco mosaic virus (MV)-infected Samsun NN tobacco.By differential colony hybridization of 1400 transformants,32 clones were obtained corresponding to TMV-inducible tobacco mRNAs. These clones were subdivided into six clusters on the basis of cross-hybridization of the inserts. By Northern blot hybridization it was shown that three of the corresponding mRNAs were strongly induced by spraying tobacco plants with salicylic acid, whereas one mRNA was weakly induced by this treatment. All mRNAs were systemically induced in plants in which only the lower leaves were locally infected by TMV. Hybrid-selected translation was performed, using six clones representing one cluster each, followed by immunoprecipitation using an antiserum to purified pathogenesis-related (PR) proteins. Four clones yielded precipitable translation products. One of these clones represented a cluster of PR-1 clones, another clone encoded the thaumatin-like (TL) protein of tobacco which may correspond to PR-P or -Q.
PubMed: 16453701
PubMed Central: PMC1167082
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.</title><author><name sortKey="Hooft Van Huijsduijnen, R A" sort="Hooft Van Huijsduijnen, R A" uniqKey="Hooft Van Huijsduijnen R" first="R A" last="Hooft Van Huijsduijnen">R A Hooft Van Huijsduijnen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, and Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, and Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen</wicri:regionArea><wicri:noRegion>6703 BD Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Van Loon, L C" sort="Van Loon, L C" uniqKey="Van Loon L" first="L C" last="Van Loon">L C Van Loon</name></author><author><name sortKey="Bol, J F" sort="Bol, J F" uniqKey="Bol J" first="J F" last="Bol">J F Bol</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1986">1986</date><idno type="RBID">pubmed:16453701</idno><idno type="pmid">16453701</idno><idno type="pmc">PMC1167082</idno><idno type="wicri:Area/Main/Corpus">000560</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000560</idno><idno type="wicri:Area/Main/Curation">000560</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000560</idno><idno type="wicri:Area/Main/Exploration">000560</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.</title><author><name sortKey="Hooft Van Huijsduijnen, R A" sort="Hooft Van Huijsduijnen, R A" uniqKey="Hooft Van Huijsduijnen R" first="R A" last="Hooft Van Huijsduijnen">R A Hooft Van Huijsduijnen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, and Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, and Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen</wicri:regionArea><wicri:noRegion>6703 BD Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Van Loon, L C" sort="Van Loon, L C" uniqKey="Van Loon L" first="L C" last="Van Loon">L C Van Loon</name></author><author><name sortKey="Bol, J F" sort="Bol, J F" uniqKey="Bol J" first="J F" last="Bol">J F Bol</name></author></analytic><series><title level="j">The EMBO journal</title><idno type="ISSN">0261-4189</idno><imprint><date when="1986" type="published">1986</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Northern (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (genetics)</term><term>Nucleic Acid Hybridization (MeSH)</term><term>Plant Proteins (genetics)</term><term>Protein Biosynthesis (MeSH)</term><term>RNA, Messenger (genetics)</term><term>RNA, Plant (genetics)</term><term>Tobacco (virology)</term><term>Tobacco Mosaic Virus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ARN des plantes (génétique)</term><term>ARN messager (génétique)</term><term>Biosynthèse des protéines (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Hybridation d'acides nucléiques (MeSH)</term><term>Protéines végétales (génétique)</term><term>Tabac (virologie)</term><term>Technique de Northern (MeSH)</term><term>Virus de la mosaïque du tabac (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Plant Proteins</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Tobacco Mosaic Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ARN des plantes</term><term>ARN messager</term><term>Protéines végétales</term><term>Virus de la mosaïque du tabac</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Cloning, Molecular</term><term>Nucleic Acid Hybridization</term><term>Protein Biosynthesis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biosynthèse des protéines</term><term>Clonage moléculaire</term><term>Hybridation d'acides nucléiques</term><term>Technique de Northern</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cDNA library was constructed to 10-15 S poly(A) RNA from tobacco mosaic virus (MV)-infected Samsun NN tobacco.By differential colony hybridization of 1400 transformants,32 clones were obtained corresponding to TMV-inducible tobacco mRNAs. These clones were subdivided into six clusters on the basis of cross-hybridization of the inserts. By Northern blot hybridization it was shown that three of the corresponding mRNAs were strongly induced by spraying tobacco plants with salicylic acid, whereas one mRNA was weakly induced by this treatment. All mRNAs were systemically induced in plants in which only the lower leaves were locally infected by TMV. Hybrid-selected translation was performed, using six clones representing one cluster each, followed by immunoprecipitation using an antiserum to purified pathogenesis-related (PR) proteins. Four clones yielded precipitable translation products. One of these clones represented a cluster of PR-1 clones, another clone encoded the thaumatin-like (TL) protein of tobacco which may correspond to PR-P or -Q.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16453701</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>18</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0261-4189</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><Issue>9</Issue><PubDate><Year>1986</Year><Month>Sep</Month></PubDate></JournalIssue><Title>The EMBO journal</Title><ISOAbbreviation>EMBO J</ISOAbbreviation></Journal><ArticleTitle>cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.</ArticleTitle><Pagination><MedlinePgn>2057-61</MedlinePgn></Pagination><Abstract><AbstractText>A cDNA library was constructed to 10-15 S poly(A) RNA from tobacco mosaic virus (MV)-infected Samsun NN tobacco.By differential colony hybridization of 1400 transformants,32 clones were obtained corresponding to TMV-inducible tobacco mRNAs. These clones were subdivided into six clusters on the basis of cross-hybridization of the inserts. By Northern blot hybridization it was shown that three of the corresponding mRNAs were strongly induced by spraying tobacco plants with salicylic acid, whereas one mRNA was weakly induced by this treatment. All mRNAs were systemically induced in plants in which only the lower leaves were locally infected by TMV. Hybrid-selected translation was performed, using six clones representing one cluster each, followed by immunoprecipitation using an antiserum to purified pathogenesis-related (PR) proteins. Four clones yielded precipitable translation products. One of these clones represented a cluster of PR-1 clones, another clone encoded the thaumatin-like (TL) protein of tobacco which may correspond to PR-P or -Q.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hooft van Huijsduijnen</LastName><ForeName>R A</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, and Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Loon</LastName><ForeName>L C</ForeName><Initials>LC</Initials></Author><Author ValidYN="Y"><LastName>Bol</LastName><ForeName>J F</ForeName><Initials>JF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>EMBO J</MedlineTA><NlmUniqueID>8208664</NlmUniqueID><ISSNLinking>0261-4189</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C053376">pathogenesis-related proteins, plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014027" MajorTopicYN="N">Tobacco Mosaic Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1986</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1986</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16453701</ArticleId><ArticleId IdType="pmc">PMC1167082</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Mol Biol. 1977 Jun 15;113(1):237-51</Citation><ArticleIdList><ArticleId IdType="pubmed">881736</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1983 Nov;25(2-3):263-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6198242</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253-7</Citation><ArticleIdList><ArticleId IdType="pubmed">6166005</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1985 Nov;4(11):2745-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16453639</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1979 Nov 24;7(6):1513-23</Citation><ArticleIdList><ArticleId IdType="pubmed">388356</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1986 May 29-Jun 4;321(6069):531-2</Citation><ArticleIdList><ArticleId IdType="pubmed">3713832</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1986 Jan;5(1):37-40</Citation><ArticleIdList><ArticleId IdType="pubmed">16453665</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1985 Sep;4(9):2167-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15938049</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Bol, J F" sort="Bol, J F" uniqKey="Bol J" first="J F" last="Bol">J F Bol</name><name sortKey="Van Loon, L C" sort="Van Loon, L C" uniqKey="Van Loon L" first="L C" last="Van Loon">L C Van Loon</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Hooft Van Huijsduijnen, R A" sort="Hooft Van Huijsduijnen, R A" uniqKey="Hooft Van Huijsduijnen R" first="R A" last="Hooft Van Huijsduijnen">R A Hooft Van Huijsduijnen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/ThaumatinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000559 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000559 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= ThaumatinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16453701
|texte= cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16453701" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ThaumatinV1
| This area was generated with Dilib version V0.6.37. |  |